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. 2016 Apr 15;36(9):1354–1365. doi: 10.1128/MCB.01004-15

FIG 1.

FIG 1

ATM kinase phosphorylates His-KIBRA at threonine 1006 site: in vitro kinase assay. (A) His-KIBRA in the presence or absence of activated ATM kinase. WB, Western blot. (B) GST–KIBRA-N (aa 1 to 453), GST–KIBRA-M (aa 428 to 835), and GST–KIBRA-C (aa 832 to 1119) as the substrates and activated ATM kinase as the enzyme. (C) Bioinformatic prediction of a plausible ATM kinase phosphorylation site in the GST–KIBRA-C (aa 832 to 1119) domain and in vitro kinase assay with GST–KIBRA-C and with GST–KIBRA-C site-specific mutants with S1003A and T1006A as the substrates and activated ATM kinase as the enzyme. In all kinase assay images, His and GST protein data are shown using a Ponceau- or mem code-stained blot illustrating equal loading of proteins, the 32P-labeling image represents phosphorylated bands, and the autoradiogram shows equal amounts of ATM kinase by Western blotting (WB).