FIG 3.

KIBRA interacts with ATM. MCF7-mCherry-KIBRA clones were treated with or without bleomycin for 2 h, and cell lysates were collected for coimmunoprecipitation. (A) Cell lysates were immunoprecipitated with ATM monoclonal antibody (Ab). The cell lysates and immunoprecipitates were analyzed by immunoblotting with antibodies as indicated. (B) Cell lysates were immunoprecipitated with polyclonal mCherry antibody. The cell lysates and immunoprecipitates were analyzed by immunoblotting with antibodies as indicated. In both cases, IgG was used as a control antibody for immunoprecipitation.