FIG 7.

KIBRA regulates DNA damage response via the NHEJ pathway. (A) MDA-MB-231 KIBRA-KD NT clones were treated with or without bleomycin for 2 h, and cell lysates were separated using SDS-PAGE and probed with the Chk2 phosphothreonine 68 antibody by Western blotting. Total Chk2 and vinculin were used as loading controls. (B) pcDNA6-FLAG and pcDNA6-FLAG-KIBRA were transiently transfected into HEK-293T cells. At 48 h posttransfection, cells were treated with bleomycin (40 μg/ml) for 2 h. Cell lysates were collected in RIPA buffer, and FLAG-KIBRA was immunoprecipitated. Eluted complexes were separated using SDS-PAGE and probed with the indicated antibodies by Western blotting. Inputs for all the indicated antibodies are shown in the right panel.