TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristics | Source or reference |
|---|---|---|
| E. coli JM109 | Cloning strain for pPD constructs | Promega |
| A. marina MBIC11017 | WT | R. Blankenshipa |
| Synechocystis strains | ||
| PCC 6803 | WT | R. Sobotkab |
| ΔbciB mutant | Emr replacement of central portion of slr1923 in WT | 11 |
| ΔbciB::nmrA(Am) mutant | AM1_2394 and Kmr replacement of psbAII in ΔbciB mutant | This study |
| ΔbciB::frhB(Am) mutant | AM1_2849 and Kmr replacement of psbAII in ΔbciB mutant | This study |
| Plasmids | ||
| pPD-FLAG | Cloning site and Kmr flanked by psbAII up- and downstream regions; Ampr | 28 |
| pPD[nmrA] | AM1_2394 with encoded His6 tag cloned into pPD-FLAG (NdeI/BglII) | This study |
| pPD[frhB] | AM1_2849 with encoded His6 tag cloned into pPD-FLAG (NdeI/BglII) | This study |
a
Departments of Biology and Chemistry, Washington University, St. Louis, MO.
b
Institute of Microbiology, Department of Phototrophic Microorganisms, Trebon, Czech Republic.