FIG 1.

Comparison of growth profiles of the WT, knockout, and complemented strains under stress conditions. (A) The knockout strains grow slowly when subjected to cold shock before being plated on MB7H9 medium containing 0.2% glycerol as a carbon source. The plate was incubated for 48 h at 37°C. (B) The initial growth of the knockout strains is slower in the carbon-starved MB7H9 medium containing only 0.02% glycerol as a carbon source. The growth of the knockout strains becomes equal to that of the WT and respective complemented strains when culture reaches mid-log phase.