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Scanning electron micrographs of planktonic cultures of relOE and dcpAOE strains and biofilm culture of WT strain of M. smegmatis. High levels of (p)ppGpp and c-di-GMP in the planktonic cultures of the strains which were shaken at 180 rpm caused the relOE and dcpAOE cells to become embedded in a biofilm-like matrix. The biofilm culture of WT M. smegmatis has been used as a control.
