TABLE 2.

List of genes selected for qRT-PCR analysis^a

Gene	Gene product	Functional classification	Fold upregulation in:
			ΔrelMsm strain	ΔdcpA strain
MSMEG_1974	Propane monooxygenase coupling protein	Virulence, detoxification, and adaptation	7.84	3.53
MSMEG_3112	Estradiol 17-beta-dehydrogenase 8	Intermediary metabolism and respiration	2.34	2.14
MSMEG_4712	TetR family protein transcriptional regulator	Cell wall and cell processes	2.96	2.12
MSMEG_4963	Pyruvate dehydrogenase E1 component, alpha subunit	Intermediary metabolism and respiration	11.64	3.06
MSMEG_5029	Peptide synthetase ScpsB, putative	Virulence, detoxification, and adaptation	3.47	2.5
MSMEG_5779	Phosphate import ATP-binding protein PstB	Cell wall and cell processes	4.51	3.4

^aRepresentative genes that were upregulated by 2-fold or more in both Δrel_Msm and ΔdcpA strains were selected for qRT-PCR analysis to validate the microarray data.