FIG 6.
Histone persistence varies depending upon the time of shutoff and the specific GAL region. Histone persistence is defined as the relative percentage of tagged histone occupancy (detected with anti-HA) over endogenous histone occupancy (detected with anti-H2B or anti-H3) under GAL gene transcriptionally repressed (“Off”) or activated (“On”) conditions in the wt and nap1Δ strains. The histone persistence at T0 was set as 100%. (A and B) H2B (A) and H3 (B) persistence at the GAL locus under transcriptionally repressed conditions. (C and D) H2B (C) and H3 (D) persistence under continuous transcriptionally active conditions. P, promoter; O, ORF. *, P value of <0.05; **, P value of <0.01.