Table 1.
Cytokines induced by phorbol 12-myristate 13-acetate/phytohaemagglutinin (PMA/PHA) treatment of Jurkat CD4+ T-lymphocytes (Mean values with their standard errors)
| Untreated control (pg/ml) | DMSO vehicle control (pg/ml) | 24 h PMA/PHA stimulated (pg/ml) | ||||
|---|---|---|---|---|---|---|
| Cytokines | Mean | sem | Mean | sem | Mean | sem |
| Eotaxin-1 | ND | 2·85 | 1·47 | 0·48 | 0·56 | |
| GM-CSF | ND | ND | 5·81* | 0·39 | ||
| IFN-γ | ND | ND | ND | |||
| IL-1β | ND | ND | ND | |||
| IL-2 | 13·45 | 1·39 | 14·40 | 1·41 | 6969·69* | 303·95 |
| IL-4 | 21·55 | 0·35 | ND | 1·86 | 5·67 | |
| IL-6 | ND | ND | ND | |||
| IL-7 | 0·3 | 0·06 | 0·80 | 0·66 | 3·52 | 0·66 |
| IL-8 | 35·25 | 2·88 | 34·22 | 2·32 | 1612·68* | 92·71 |
| IL-12p70 | ND | ND | ND | |||
| IL-13 | ND | ND | 7·68* | 1·52 | ||
| MCP-1 | ND | ND | ND | |||
| TNFα | ND | ND | 259·55* | 12·36 | ||
GM-CSF, granulocyte macrophage-colony stimulating factor; IFN, interferon; MCP, monocyte chemoattractant protein.
*
P<0·05 compared with DMSO vehicle control.