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BAL fluid antiviral activity was sensitive to sialidase. Shown are HI titers from 12 BAL fluid samples against MVCU-013/20099(pdmH1N1). The BAL fluid samples were subjected to the following treatments prior to the assay: heat treatment at 56°C and exposure to the receptor-degrading enzyme (RDE), CaCl2, EDTA, and protease. HI titers are shown as the geometric mean with 95% CI (***, P < 0.001; t test).
