FIG 7.

IgA and IgG depletion. IgA and IgG were depleted from one BAL fluid sample using the IgA-binding lectin jacalin and protein G beads, respectively. (A) After the depletion, the samples were analyzed by Western blotting using human IgA- and IgG-specific antibodies. The IgA and IgG heavy chains have molecular masses of approximately 58 to 80 kDa. (B) HI titers of IgA- and IgG-depleted BAL fluid against Siriraj-04/2003(H3N2), Siriraj-03/2006(sH1N1), and 104/2009(pdmH1N1) were comparable to those of untreated BAL fluid. The data were derived from 3 BAL fluid samples and run in duplicate. HI titers are shown as the geometric mean with 95% CI (**, P = 0.0075; ***, P = 0.0009; t test).