FIG 1.

Genetic map of HBoV1 and the probes used. (A) The expression profiles of HBoV1 mRNA transcripts and their encoded proteins are shown with transcription landmarks and boxed ORFs. The numbers are nucleotide numbers of the HBoV1 full-length genome (GenBank accession no. JQ923422). Major species of HBoV1 mRNA transcripts that are alternatively processed are shown with their sizes in kilobases [minus a poly(A) tail of ∼150 nt], which were derived from multiple studies of HBoV1 DNA transfection of HEK 293 cells and HBoV1 infection of polarized human airway epithelium (12, 16, 17). Viral proteins detected in both transfected and infected cells are shown side by side on the right. P5, P5 promoter; D, 5′ splice donor site; A, 3′ splice acceptor site; (pA)p and (pA)d, proximal (internal) and distal polyadenylation sites, respectively; LEH/REH, left-end/right-end hairpins. (B) Probes used for Northern blot analysis with nucleotide numbers. The probes used for the RPA are diagrammed with the sizes of their detected bands.