FIG 4.
Reconstruction of D5323–735 from negative-stain EM. (A) (Left) Reference-free class averages of the views from negative-stain EM; (right) the best-matched projections from the reconstructed model. (B) Top, side, and bottom views of the EM reconstruction, from top to bottom, respectively. The contour level of the envelope has been set to correspond to the expected volume of the D5323–735 hexamer. (C) Comparison of the EM envelope (cyan) with the crystallographic model of the SV40 Lta hexamer (cartoon representation in green). (D) Comparison of the EM envelope (cyan) contoured at the contour level that gives the same volume as the ab initio bead model derived from SAXS (blue), which is superposed. (E) Theoretical scattering curve (blue) based on the envelope shown in panel D compared with the experimental scattering curve from SAXS (black).