TABLE 1.
Construct | Rg from Guinier plot (nm) | Reciprocal space Rg (nm)b | Dmax (nm)b | Porod vol (103 Å3) | Molecular mass (kDa) calculated from: |
|
---|---|---|---|---|---|---|
Porod volc | Sequence | |||||
D51–335 | 2.9 | 2.9 | 11 | 65 | 39 | 39.0 |
D5323–785 | 4.8 | 4.7 | 14.5 | 570 | 345 | 53.5 × 6 (321) |
D5381–785 | 2.8 | 2.8 | 9 | 80 | 48 | 46.7 |
Absolute uncertainties for Rg from the Guinier plot, the reciprocal space Rg, and Dmax were ±0.05 nm, ±0.1 nm, and ±0.4 nm. The relative uncertainties of the Porod volume and the molecular mass from the Porod volume were 5%.
Calculated from the P(r) function.
Calculated according to reference 20.