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. 2016 Apr 14;90(9):4346–4356. doi: 10.1128/JVI.00179-16

FIG 6.

FIG 6

HCMV IE2 overexpression rescues HCMV gene expression but not virion production in TAg-expressing fibroblasts. (A) Lysates of primary fibroblasts infected with HCMV (MOI = 3.0) at 24 hpi PDGFRα-expressing TAg cells transduced with either the HCMV MIE gene or a control vector were subjected to Western blotting analysis of IE2. (B) Primary fibroblasts and PDGFRα-expressing TAg cells transduced with the HCMV MIE gene or a control vector were infected with HCMV (AD169, MOI = 3.0) and harvested at 4, 24, 48, and 72 hpi. The resulting lysates were subjected to Western blotting analysis of IE1, IE2, UL26, UL44, pp28, and GAPDH. The numbers to the left of the gels in panels A and B are molecular masses (in kilodaltons). (C) The viral DNA abundance in the cells infected as described in the legend to panel B was quantified and is displayed as the copy number per nanogram of input DNA on a log10 scale. (D) PDGFRα-expressing TAg cells transduced with the HCMV MIE gene or a control vector were infected with HCMV (AD169, MOI = 3.0). Production of infectious virions was measured by plaque assay at 120 hpi.