TABLE 1.

Primers used in DENV protein cloning or mutagenesis

Purpose	Name	Sequence
Individual DENV2 protein expression
Core	F-EcoRI	5′-GGAATTCATGATGAATAACCAACGGAAAAAGGCG-3′
	R-NotI	5′-AAGGAAAAAAGCGGCCGCCCGCCATCACTGTTGGAATCAGCAT-3′
NS1	F-NotI	5′-AAGGAAAAAAGCGGCCGCATGGATAGTGGTTGCGTTGTGAGCTGG-3′
	R-KpnI	5′-GGGGTACCCGGCTGTGACCAAGGAGTTGACCAA-3′
NS2A	F-EcoRI	5′-GGAATTCATGGGACATGGGCAGATTGACAACTTC-3′
	R-NotI	5′-AAGGAAAAAAGCGGCCGCCCCTTTTCTTGCTAGTTCGTGAAAG-3′
NS2B	F-EcoRI	5′-GGAATTCATGAGCTGGCCACTAAATGAGGCTATC-3′
	R-NotI	5′-AAGGAAAAAAGCGGCCGCCCCGTTGTTTCTTCACTTCCCACAG-3′
NS3	F-NotI	5′-AAGGAAAAAAGCGGCCGCATGGCTGGAGTATTGTGGGATGTCCCT-3′
	R-KpnI	5′-GGGGTACCCACTTTCTTCCAGCTGCAAATTCCTT-3′
NS4A	F-EcoRI	5′-GGAATTCATGTCCTTGACCCTGAACCTAATCACA-3′
	R-NotI	5′-AAGGAAAAAAGCGGCCGCCTCTTTTCTGAGCTTCTCTAGTTGC-3′
NS4B	F-EcoRI	5′-GGAATTCATGGCAGCAGCGGGCATCATGAAAAAC-3′
	R-NotI	5′-AAGGAAAAAAGCGGCCGCCCCTTCTTGTATTGGTTGTGTTCTT-3′
NS5	F-NotI	5′-AAGGAAAAAAGCGGCCGCATGGGAACTGGCAACATAGGAGAGACG-3′
	R-NotI	5′-AAGGAAAAAAGCGGCCGCCCCACAGGACTCCTGCCTCTTCCTC-3′
DENV2 NS5 truncation mutants
N1	F-1∼627	5′-AAGGAAAAAAGCGGCCGCATGGGAACTGGCAACATAGGAGAGACG-3′
	R-1∼627	5′-AAGGAAAAAAGCGGCCGCCTCCTTCTCCTTCCATCTGTCT-3′
N2	F-1∼450	5′-AAGGAAAAAAGCGGCCGCATGGGAACTGGCAACATAGGAGAGACG-3′
	R-1∼450	5′-ACCCGGGACTCGAGGCGGCCGCACATGTTTCACACTTTCCTTC-3′
C1	F-71∼900	5′-AAGGAAAAAAGCGGCCGCATGGTCACACCAGAAGGGAAAGTA-3′
	R-71∼900	5′-AAGGAAAAAAGCGGCCGCCCCACAGGACTCCTGCCTCTTCCTC-3′
C2	F-301∼900	5′-AAGGAAAAAAGCGGCCGCATGACATGGGCTTACCATGGTAGC-3′
	R-301∼900	5′-AAGGAAAAAAGCGGCCGCCCCACAGGACTCCTGCCTCTTCCTC-3′
C3	F-301∼705	5′-AAGGAAAAAAGCGGCCGCATGACATGGGCTTACCATGGTAGC-3′
	R-301∼705	5′-AAGGAAAAAAGCGGCCGCCTTGTGTCCAGTCGCTCCACCC-3′
C4	F-301∼545	5′-AAGGAAAAAAGCGGCCGCATGACATGGGCTTACCATGGTAGC-3′
	R-301∼545	5′-AAGGAAAAAAGCGGCCGCCTTCCAGTGTGATTCTTGTGTC-3′
DENV2 NS5 K-R substitution
K139R	F	5′-TTCTTTACTCCGCCAGAAAGGTGCGACACATTGCT-3′
	R	5′-AGCAATGTGTCGCACCTTTCTGGCGGAGTAAAGAA-3′
K181R	F	5′-TGCATAAGGGTTCTCAACCCATACATGCCC-3′
	R	5′-GAGAACCCTTATGCAAAATTGGATGTTGTTGTT-3′
K193R	F	5′-CCATACATGCCCTCAGTCATAGAAAGAATGGAAGCACTAC-3′
	R	5′-GTAGTGCTTCCATTCTTTCTATGACTGAGGGCATGTATGG-3′
K200R	F	5′-GGAAGCACTACAAAGGAGATATGGAGGAGCCTTAG-3′
	R	5′-CTAAGGCTCCTCCATATCTCCTTTGTAGTGCTTCC-3′
K285R	F	5′-GAAAAAATAAGACAAGAGCATGAAACATCATG-3′
	R	5′-TCTTATTTTTTCTATTCTTTTCCCAATTATGTCCAG-3′
K101/105R	F	5′-GAAGTCAGAGGCCTGACAAGAGGA-3′
	R	5′-CAGGCCTCTGACTTCTCTTACATT-3′
K246/248/249R	F	5′-ATGAGACACAGGAGAGCCACTTACGAGCCA-3′
	R	5′-GGCTCTCCTGTGTCTCATTGTGAATCTATTG-3′
K279/283/285R	F	5′-GGGAGAAGAATAGAAAGAATAAGACAAGAGCAT-3′
	R	5′-TCTTATTCTTTCTATTCTTCTCCCAATTATGTCCAG-3′
SIMmut	F	5′-AAAGCAGCGGCCGCTGGTTGCGGCAGAGGA-3′
	R	5′-ACCAGCGGCCGCTGCTTTCCCTTCTGGTGT-3′