FIG 4.

GBP1 does not activate the IFN-β pathway. (Top) HEK293T cells cotransfected with pHA-GBP1 or pCMV-HA (pHA-EV) plus pIFN-β-Fluc and pTK-Rluc (A), pISRE-Fluc and pTK-Rluc (B), or pNF-κB-Fluc and pTK-Rluc (C) for 24 h were either left untreated or treated with 20 HAU/ml SeV for 24 h and were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega). pTK-Rluc was used as an internal reference. Each sample was run in triplicate. Error bars represent standard deviations. HA, hemagglutinin tag. (Bottom) The expression of HA-GBP1 or HA-EV (HA empty vector) in HEK293T cells was determined by Western blotting (WB). GAPDH was used as a loading control.