TABLE 1.
Primers used in this study
| Primer | Sequence (5′–3′) | Usage |
|---|---|---|
| HA-GBP1-F | GCGTCGACCATGGCCTCAAAGGTGCACATG | Amplification of GBP1 |
| HA-GBP1-R | GAAGATCTTTAGCTCAGGAAACATTCTTT | |
| Flag-GBP1-F | GCCGATATCGATGGCCTCAAAGGTGCACATGC | Amplification of GBP1 |
| Flag-GBP1-R | CGCGGATCCTTAGCTCAGGAAACATTCTTTC | |
| GST-GBP1-F | CGGGATCCATGGCCTCAAAGGTGCACATGC | Amplification of GBP1 |
| GST-GBP1-R | CCGCTCGAGTTAGCTCAGGAAACATTCTTTC | |
| Flag-GBP1(R48P)-F | TTGTGGGCCTGTACCCCACAGGCAAATCCTAC | Amplification of GBP1(R48P) |
| Flag-GBP1(R48P)-R | GTAGGATTTGCCTGTGGGGTACAGGCCCACAA | |
| Flag-GBP1(K51A)-F | GTACCGCACAGGCGCATCCTACCTGATGAAC | Amplification of GBP1(K51A) |
| Flag-GBP1(K51A)-R | GTTCATCAGGTAGGATGCGCCTGTGCGGTAC | |
| Flag-GBP1(1-308)-F | CGGAATTCAATGGCCTCAAAGGTGCACATG | Amplification of GBP1(1-308) |
| Flag-GBP1(1-308)-R | CGGGATCCTTAGCAGGGCAGGTCCCCAC | |
| Flag-GBP1(309-591)-F | GCCGATATC GATGGAGAATGCAGTCCTGGC | Amplification of GBP1(309-591) |
| Flag-GBP1(309-591)-R | CGCGGATCCTTAGCTCAGGAAACATTCTTTC | |
| Myc-NS5A-F | CCGGAATTCGGATGTCAAGTAATTACATTCTAGAGC | Amplification of NS5A |
| Myc-NS5A-R | CCGCTCGAGTCACAGTTTCATAGAATACAC | |
| Myc-NS5A(1-268)-F | CCGGAATTCGGATGTCAAGTAATTACATTCTAGAGC | Amplification of NS5A(1-268) |
| Myc-NS5A(1-268)-R | CCGCTCGAGTCAAGCAGGCTGCAAGGTTATCTC | |
| Myc-NS5A(269-497)-F | CCGGAATTCGGATGGTAGTGGTGGATACAAC | Amplification of NS5A(269-497) |
| Myc-NS5A(269-497)-R | CCGCTCGAGTCACAGTTTCATAGAATACAC | |
| pFUGW-sfiI(A)-GBP1-F | ACAGGCCATTACGGCCATGGCCTCAAAGGTGCA | Amplification of GBP1 |
| pFUGW-sfiI(B)-GBP1-R | TACGGCCGAGGCGGCCTTATTAGCTCAGGAAACATT | |
| Q-GBP1-F | GAAGGGTGACAACCAGAACGAC | qRT-PCR for detection of GBP1 |
| Q-GBP1-R | AGGTTCCGACTTTGCCCTGATT | |
| Q-GAPDH-F | GAAGGTCGGAGTGAACGGATTT | qRT-PCR for detection of GAPDH |
| Q-GAPDH-R | TGGGTGGAATCATACTGGAACA |