FIG 6.
The inhibition of HDAC activity inhibits the phosphorylation of STAT1 and expression of ISGs. (A to E) A549 cells (8 × 105) were infected with PR8 at an MOI of 0.5 and subsequently treated with DMSO or the indicated concentrations of TSA (in DMSO) for 24 h. Total cell lysates were prepared, and phosphorylated STAT1 (pSTAT1, 91/84 kDa), along with total STAT1 (tSTAT1) as a loading control (A), and IFITM3 (15 kDa) (B), ISG15 (15 kDa) (C), or viperin (42 kDa) (D), along with PDI as loading control, were detected in uninfected (UNI) and infected (INF) cell lysates by WB. (E) In the same lysates, Acet H3, total H3, and NP were detected by WB as the markers of TSA potency, loading control, and infection, respectively. MW, molecular weight.