FIG 3.

Dose titration of CPPD and M-Tri-DAP. Adult female CB6F1 mice were immunized i.m. with 0.1 μg H1N1 antigen alone or in combination with a 2.5-, 10-, or 40-μg dose of CPPD (A to E) or a 0.05-, 0.5-, or 5-μg dose of M-Tri-DAP (F to J) prior to infection with H1N1 influenza virus. (A and F) Serum anti-H1N1 IgG levels were assessed before infection. (B and G) Mice were monitored for weight changes during infection. Influenza virus M gene RNA levels (C and H) and levels of CD8⁺ cells specific for influenza virus in lung (D and I) and BAL fluid anti-H1N1 IgA levels (E and J) were determined on day 7 after infection. Lines and points represent means of the results determined with n = ≥4 mice. *, P < 0.05; **, P < 0.01 (measured by one-way ANOVA [A] or two-way ANOVA [G]).