FIG 5.

Properties of microparticle adjuvants and effect on immune response. (A and B) Light microscopic imaging of suspensions of NanoSiO2 (A) or CPPD (B). (C) Analysis of protein adsorption by microparticles. Lines represent means of n = 4 studies. (D to H) Adult female CB6F1 mice were administered PBS, 1 μg MPLA, MF59, 40 μg CPPD, or 5 μg M-Tri-DAP i.m. in a 50-μl volume in the right hind quadriceps muscle. Serum was sampled at 4, 12, and 24 h after injection, and serum CCL2 (D), CXCL1 (E), IL-5 (F), IL-1β (G), and IL-1α (H) levels were determined by Luminex assay. (I to M) Mice were culled for harvest at 24 h, and injected muscles were excised and digested into a single-cell suspension for analysis by flow cytometry. (I to K) Live cells were stained for CD11b and Ly6G (I), F4/80 (J), and CD11c/MHCII (K). (L and M) CD80 percentages on F4/80 (L) and CD11c cells (M) were measured by flow cytometry. Lines in panels C and I through M and points in panels D to H represent means of results determined with n > 4 mice. *, P < 0.05; **, P < 0.001 (measured by Kruskal Wallis test).