FIG 10.

Binding of MAbs 17b, CG10, and N12-i15 to FPLC-purified monomeric gp120. The gp120 monomers of the truncations described in the legends of Fig. 3B, 7, and 9 were purified by FPLC and tested by an ELISA for MAb binding in the presence and absence of CD4, as indicated. Binding to purified monomeric full-length BaL gp120 is given for comparison. Patterns of binding to monomeric gp120 were identical to the patterns of binding to mixed monomeric and oligomeric preparations. Statistically significant differences (P < 0.05) between the “−sCD4” and “+sCD4” columns are marked with an asterisk.