FIG 6.
V3 loop modifications affect binding by relaxed CD4i MAbs. A binding ELISA was carried out to ascertain the effects of V3 loop truncations on binding by the CD4i MAb panel to gp120BaL. Full truncation of the V3 loop (ΔV3) results in stringent binding by MAbs 17b and 21c, an effect not seen for the V3 base mutant. Binding by the stringent CD4i MAbs CG10, 19e, and N12-i15 was not affected by V3 loop truncations. Statistically significant differences (P < 0.05) between the “−sCD4” and “+sCD4” columns are marked with an asterisk.