FIG 1.

Characterization of infection of Vero, SUM159, SVG-A, and A549 cells by JUNV. (A, B) JUNV (MOI, 0.1) was inoculated onto Vero, SUM159, SVG-A, or A549 cells for 1 h at 37°C, and the cells were subsequently washed and reincubated for the indicated times postinfection. Cells were fixed and stained with an anti-NP antibody coupled to A647, and the percentage of infected cells (A) and the A647 mean fluorescence (fluo.) intensity of the infected cells (B) were measured by flow cytometry. The data correspond to the means ± SDs from duplicate experiments with at least 10,000 cells per condition. (C, D) Vero, SUM159, SVG-A, or A549 cells infected with JUNV (MOI, 0.03) for 1 h were subsequently washed extensively and reincubated in virus medium for 24 h at 37°C. The supernatant was removed, and fresh medium was added to the cells for 48 h (3 days postinfection). The supernatant was collected, and fresh medium was added for an additional 48 h (5 days postinfection). The supernatant was collected, and plaque assays were performed to quantify the number of infectious particles released by the different cell types between days 1 and 3 postinfection (C) and days 3 and 5 postinfection (D). The data correspond to the means ± SDs from duplicate experiments. (E) Vero, SUM159, SVG-A, or A549 cells treated as described in the legends to panels C and D were used to assess cell viability using the CellTiter-Glo viability assay with noninfected cells (black line) or JUNV-infected cells (blue line). The luminescence was measured using the same luminometer parameters for each cell type, and the results are expressed as arbitrary luminescence units. The data correspond to the means ± SDs from triplicate experiments.