FIG 9.

The antiviral activity of ZCL278 extends to other enveloped viruses. Vero (A, B, D) or Huh7 (C) cells pre- and cotreated in the presence or absence of 50 μM ZCL278 as described in the legend to Fig. 3D were infected with VSV for 6 h at an MOI of 0.3 (A), LCMV for 16 h at an MOI of 0.3 (B), DV2 for 24 h at an MOI of 1 (C), or PV1 for 6 h at an MOI of 1 (D). The percentage of VSV- and LCMV-infected cells detected by virus-specific antibody staining was measured by flow cytometry (A, B). The amount of infectious DV2 particles released was measured by plaque assay (C), and the replication of PV1 was evaluated by RT-qPCR (D). For all histograms, the values presented are relative to those for the untreated control. Error bars represent SDs from experiments performed at least in duplicate.