FIG 2.
Images of the TfR-parvovirus binding experiments at different stages. (A) Images of the Alexa 594-labeled virus adsorbing to glass after 30 min of incubation. White numbers are the particle counts, Pcount. In this panel, Pcount is equal to Vglass, which is used to quantify the relative virus concentrations between virus batches. Pcount is shown instead of N or N+ because Pcount is insensitive to the binding event criteria. (B) Images of SLBs incubated with virus after a 10-min waiting period. Negligible binding events here indicate that the viruses do not readily bind to the SLBs and that the SLBs have no defects exposing the glass surface. (C) Images of the eGFP-labeled TfR attached to the SLBs, prior to loading the virus. Yellow numbers are the TfR particle counts, RTfR. (D) Images of parvovirus binding to TfR-loaded SLBs after 10 min. Image details: each image portrays an 82- by 82-μm2 physical space in 512 by 512 pixels. Uneven backgrounds were subtracted out, and all image intensities were linearly scaled, which preserves particle features.