FIG 4.

Relative susceptibility of domestic dog and raccoon cells to infection by Rac118-300D and -300G. (A) Dog (A72) and raccoon (Pl1Ut) cells were infected with equivalent amounts of Rac118-300D or -300G and fixed, stained, and analyzed by immunofluorescence at 72 h postinfection using a polyclonal rabbit anti-CPV VP1/VP2 antibody and a goat anti-rabbit Alexa Fluor 488 secondary antibody. (B) For relative infectivity estimates, Alexa Fluor 488-positive cells were visually counted and the number of infected cells seen with the normal VP2 position 300 mutation associated with each host (i.e., Asp in raccoons, Gly in dogs) was set to 1.0. Error bars indicate standard deviations for duplicate experiments.