Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
MK886 inhibits FLAP-mediated increase of 5-LOX product formation in intact cells. HEK293 cells expressing 5-LOX-wt or mutants with or without FLAP were preincubated with 30 nM MK886 or vehicle (0.1% DMSO) for 15 min at 37°C before stimulation with 2.5 µM A23187 plus 3 µM AA for another 10 min at 37°C. 5-LOX products were analyzed by reverse-phase HPLC. Data are expressed as means ± sem; n = 3–5, duplicates; ***P < 0.001; 30 nM MK886 vs. vehicle (DMSO) control, Student’s t test.
