Figure 2.

Down-regulation of Nrf2-regulated antioxidant gene expression in the CTX-injured regenerating TA muscles of Nrf2^−/− mice. Transcript levels of Nrf2 (A), Gclc (B), Gclm (C), Nqo1 (D), G6pd (E), Gsr (F), Sod1 (G), and Sod2 (H) were determined in WT and Nrf2^−/− TA muscle by quantitative RT-PCR. The relative gene expression was calculated by normalizing the mRNA levels of gene of interest with the levels of housekeeping gene, Gapdh. n = 4/d per group. Statistical significance was calculated by 1-way ANOVA. ^#P < 0.05 PBS vs. CTX-WT, *P < 0.05 CTX-WT vs. Nrf2^−/−.