Figure 3.

Poor recovery of Nrf2-regulated antioxidant protein levels in regenerating TA muscles of Nrf2^−/− mice injured with CTX. A–D) Protein levels of Nrf2-regulated antioxidants (catalase, NQO1, GCLC, G6PD, SOD1, and SOD2) were determined by Western blot analysis of tissues homogenates of regenerating TA muscles collected after CTX-mediated injury and regeneration on d 2 (A), d 4 (B), d 8 (C), and d 15 (D). E) Western blots for GSR investigating changes in its levels during post-CTX injury regeneration of TA muscle of WT and Nrf2^−/−. F–L) ImageJ densitometry quantification for NQO1 (F), GCLC (G), G6PD (H), SOD1 (I), SOD2 (J), GSR (K), and catalase (L) immunoblots. n = 4/d per group. Statistical significance was calculated by 1-way ANOVA. ^#P < 0.05 PBS vs. CTX-WT, *P < 0.05 CTX-WT vs. Nrf2^−/−.