Figure 4.

Nrf2-deficient mice displayed increased levels of ROS in CTX-injured regenerating TA muscle. A, B) Frozen TA muscle sections from control and CTX-treated WT and Nrf2^−/− mice were loaded with fluorescent ROS probes: DHE (A) and chloromethyl derivative of 2′7′-dichlorodihydrofluorescein diacetate (CM-DCFDA) (B) were processed for fluorescent microscope (×40 magnification) analysis. C, D) The mean fluorescence intensity of DHE (red fluorescence) (C) and CM-DCFDA (green fluorescence) (D) were measured by ImageJ software; n = 4/d per group. Statistical significance was calculated by 1-way ANOVA. ^#P < 0.05 PBS vs. CTX-WT, *P < 0.05 CTX-WT vs. Nrf2^−/−.