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. Author manuscript; available in PMC: 2016 Apr 19.
Published in final edited form as: Methods Enzymol. 2011;497:295–372. doi: 10.1016/B978-0-12-385075-1.00014-7

Figure 14.11.

Figure 14.11

Comparison of phase contrast and differential interference contrast (DIC) imaging with regards to cell tracking. (A) DIC image of an E. coli colony growing in a microchemostat device. (B) Phase contrast imaging of a similarly grown E. coli colony. (C) Binary image created by thresholding the DIC image shown in part A. Notice how difficult it is to distinguish the cellular boundaries. (D) Thresholded version of the phase contrast image in part B. Notice how much more clearly the cellular boundaries are compared to C.

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