FIG 2.

Time course of ALA production by recombinant C. glutamicum strains. Wild-type C. glutamicum and CgS1 harboring the ALAS of R. sphaeroides 2.4.1 were cultured in a 300-ml baffled shake flask containing 50 ml modified CGXII medium, at 30°C and 180 rpm, and the pH was maintained at approximately 6.5. The initial glucose (Glc) concentration was 40 g/liter. The precursor glycine (Gly) was added at 2 g/liter every 12 h, and 10 g/liter succinate (Suc) was added when needed. Results are averages from three independent experiments, with standard deviations indicated by error bars.