TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s) | Source |
|---|---|---|
| Strains | ||
| E. coli DH5α | Wild type; subcloning host | TransGen Biotech |
| C. glutamicum ATCC 13032 | Wild type; biotin auxotrophic | ATCC |
| C. glutamicum CgS1 | C. glutamicum ATCC 13032 ΔsucCD | This study |
| C. glutamicum CgS1-ASV | ASV tag added to C terminus of ALAD in CgS1 | This study |
| Plasmids | ||
| pEC-XK99E | Kanr; E. coli-C. glutamicum shuttle vector | Laboratory stock |
| pEC-RS | Kanr; pEC-XK99E carrying gene for ALAS from R. sphaeroides 2.4.1 | This study |
| pEC-SB | Kanr; pEC-XK99E carrying gene for ALAS from R. capsulatus SB1003 | This study |
| pEC-GS | Kanr; pEC-XK99E carrying gene for ALAS from P. pastoris GS115 | This study |
| pEC-SC | Kanr; pEC-XK99E carrying gene for ALAS from S. cerevisiae s288c | This study |
| pEC-AT | Kanr; pEC-XK99E carrying gene for ALAS from A. tumefaciens C58 | This study |
| pEC-SB-gltA | pEC-SB carrying citrate synthase (gltA) from C. glutamicum ATCC 13032 additionally | This study |
| pEC-SB-icd | pEC-SB carrying isocitrate dehydrogenase (icd) from C. glutamicum ATCC 13032 additionally | This study |
| pEC-SB-odhA | pEC-SB carrying 2-oxoglutarate dehydrogenase (odhA-sucB-lpd) from C. glutamicum ATCC 13032 additionally | This study |
| pEC-SB-coaA | pEC-SB carrying pantothenate kinase (coaA) from C. glutamicum ATCC 13032 additionally | This study |
| pEC-SB-rhtA | pEC-SB carrying ALA exporter (rhtA) from E. coli MG1655 additionally | This study |
| pEC-SB-rhtA-coaA | pEC-SB carrying rhtA and coaA additionally | This study |
| pK-JL | pK19mobsacB derivative | 23 |
| pK-JLΔsucCD | Kanr; containing flanking regions of C. glutamicum sucCD genes | This study |