TABLE 2.
Determination of TCA intermediate levels in wild-type C. glutamicum and CgS1a
| Strain | Intermediate level (g/liter) |
||||||
|---|---|---|---|---|---|---|---|
| Citrate | Isocitrate | α-Ketoglutarate | Succinate | Fumarate | Malate | Glyoxylate | |
| Wild type | 1.25 ± 0.09 | 1.62 ± 0.21 | 0.63 ± 0.11 | 0.13 ± 0.03 | 0.15 ± 0.02 | 0.22 ± 0.03 | 0.34 ± 0.02 |
| CgS1 | 1.16 ± 0.1 | 1.42 ± 0.13 | 0.54 ± 0.09 | 0.13 ± 0.02 | 0.16 ± 0.02 | 0.25 ± 0.01 | 0.36 ± 0.01 |
a
Wild-type C. glutamicum and CgS1 strains were cultured in modified CGXII medium for 24 h, and fermentation supernatants were analyzed for related intermediates.