FIG 5.

Ammonium transporters of “Ca. Nitrosotalea devanaterra.” (A) Maximum-likelihood phylogeny of Amt/Rh transporters. The transported substrate (ammonia versus ammonium) has been validated in organisms highlighted with red and blue circles. Red, ammonium (NH₄⁺); blue, ammonia (NH₃). Circles at nodes highlight the level of bootstrap support, and the scale bar represents 0.5 change per amino acid position. (B) Conservation of the cation binding site in Amt/Rh sequences. (C) Transport mechanisms of Amt/Rh transporters previously reported in the literature (see Table S9 in the supplemental material).