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. 2016 Apr 18;82(9):2620–2631. doi: 10.1128/AEM.03851-15

TABLE 1.

Primers used for microbial identification in molecular tests

Primer Sequence Region Reference(s)
Fungal primers
    ITS1F 5′-CTTGGTCATTTAGAGGAAGTAA-3′ ITS1 27
    ITS1F-GC 5′-CGCCCGCCGGCGGCGGCGGGCGGGGCGGGGGCA-CGGGGGGCTTGGTCATTTAGAGGAAGTAA-3′ ITS1 27
    ITS1 5′-TCCGTAGGTGAACCTGCGG-3′ ITS1 28, 29
    ITS2 5′-GCTGCGTTCTTCATCGATGC-3′ ITS1 28
    ITS4 5′- TCCTCCGCTTATTGATATGC-3′ ITS1 28, 29
Bacterial primers
    U968 5′-AACGCGAAGAACCTTAC-3′ 16S rRNA gene 30
    L1401 5′-CGGTGTGTACAAGACCC-3′ 16S rRNA gene 30