FIG 2.
Analysis of mutants defective for the loci from 2292 to 2295. (A) Diagram of the ORFs surrounding 2292 to 2295, with the locations of transposon-insertion mutations 10E8 and 3C6 to 3C9 indicated by red arrowheads. (B) Photographs of plaque plates taken 7 days following addition of amoebae on cultures of the WT, 2292−, 2293−, and related expression strains. The strain on each plate is identified by a combination of column and row labels as in Fig. 1. (C) Silver-stained Tris-glycine SDS gel of outer membrane preparations of the WT, 2292−, 2293−, and expression strains labeled as in Fig. 1, with the region containing bands representing O antigen attached to a lipid anchor (OAL) indicated on the left and molecular mass standards, in kilodaltons, on the right. ---, WT NS1; 92, 2292cx; 93, 2293cx. (D) Plaque plates of 3C7, 3C9, and controls photographed 7 days following amoeba addition. (E) Outer membrane analysis of 3C7 and 3C9, labeled as in panel C.