FIG 4.
Enhancement of IL-10 production and Treg cells in mice orally administered the extract of B. rapa L. Sampling of spleen cells was conducted in the same manner as described in the legend of Fig. 3. (a) Spleen cells (5 × 105 cells/well) from mice were stimulated with LPS (0.1 and 1 μg/ml) for 48 h. IL-10 production levels in the supernatants were measured by ELISA. (b) Spleen cells from mice orally administered the extract of B. rapa L. (20 mg/kg of BW/day) or water were stained with anti-CD4, anti-CD25, and anti-Foxp3 MAbs, and the percentages of Treg cells were evaluated by flow cytometry. Data are presented in the same manner as described in the legend to Fig. 1.