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. 2015 Dec 9;5(1):53–64. doi: 10.1080/21623945.2015.1119957

Figure 5.

Figure 5.

Effects of HCMV infection on adipogensis in ASCs (A) ASCs were cultured in CCM or AIM media for 14 d and mock-infected or infected with HCMV TR strain at MOI = 1. Lipid droplet accumulation was detected using Oil red O staining solution. Images were captures using brightfield settings on an inverted microscope. (B) ASCs were cultured in CCM or AIM media for 14 d and mock-infected or infected with HCMV TR strain at MOI = 1. Lipid droplet accumulation was detected using BODIPY staining solution. Images were captures using a fluorescent inverted microscope. Scale bars = 10 μm. (C) Analysis of ASC mock-infected or infected with TR for 14 d at a MOI = 1. Cellular mRNA was processed for analysis on a custom RT-PCR array designed to examine adipogenic related genes. Fold change of infected cells is relative to mock-infected cells after normalization to house-keeping genes (GAPDH and TBP). Data is from one representative donor out of 3 independent donors. (D) Analysis of ASC mock-infected or infected and then cultured in differentiation media with HCMV TR for 14 d at a MOI = 1. Cellular mRNA was processed for analysis on a custom RT-PCR array designed to examine adipogenic related genes. Fold change of infected cells is relative to mock-infected cells after normalization to housekeeping genes. Data is from one representative donor out of 3 independent donors.