Table 1.
Target group | Primer sequence (5′-3′) | Genomic DNA Standard | PCR product Size (bp) | AT | Reference |
---|---|---|---|---|---|
Firmicutes | ATG TGG TTT AAT TCG AAG CA AGC TGA CGA CAA CCA TGC AC |
Lactobacillus gasseri ATCC 33323 |
126 | 60°C | 38 |
Bacteroidetes | CAT GTG GTT TAA TTC GAT GAT AGC TGA CGA CAA CCA TGC AG |
Bacteroides vulgatus ATCC 8482 |
126 | 60°C | 38 |
Lactobacillus | GAG GCA GCA GTA GGG AAT CTT C GGC CAG TTA CTA CCT CTA TCC TTC TTC |
Lactobacillus gasseri ATCC 33323 |
126 | 60°C | 39 |
Enterococcus | CCC TTA TTG TTA GTT GCC ATC ATT ACT CGT TGT ACT TCC CT TGT |
Enterococcus gilvus ATCC BAA-350 |
144 | 61°C | 40 |
Clostridium leptum | GCA CAA GCA GTG GAG T CTT CCT CCG TTT TGT CAA |
Clostridium leptum ATCC 29065 |
239 | 60°C | 41 |
Bacteroides | ATA GCC TTT CGA AAG RAA GAT CCA GTA TCA ACT GCA ATT TTA |
Bacteroides vulgatus ATCC 8482 |
495 | 60°C | 42 |
Prevotella | CAC RGT AAA CGA TGG ATG CC GGT CGG GTT GCA GAC C |
Prevotella nigrescens ATCC 33563 |
513 | 55°C | 42 |
Bifidobacterium | CGC GTC YGG TGT GAA AG CCC CAC ATC CAG CAT CCA |
Bifidobacterium longum subsp. Infantis ATCC 15697 | 244 | 60°C | 39 |
AT, annealing temperature; bp, base pairs.