Table 1.

Primer sequences and real-time PCR conditions used for gut microbiota analysis

Target group	Primer sequence (5′-3′)	Genomic DNA Standard	PCR product Size (bp)	AT	Reference
Firmicutes	ATG TGG TTT AAT TCG AAG CA AGC TGA CGA CAA CCA TGC AC	Lactobacillus gasseri ATCC 33323	126	60°C	38
Bacteroidetes	CAT GTG GTT TAA TTC GAT GAT AGC TGA CGA CAA CCA TGC AG	Bacteroides vulgatus ATCC 8482	126	60°C	38
Lactobacillus	GAG GCA GCA GTA GGG AAT CTT C GGC CAG TTA CTA CCT CTA TCC TTC TTC	Lactobacillus gasseri ATCC 33323	126	60°C	39
Enterococcus	CCC TTA TTG TTA GTT GCC ATC ATT ACT CGT TGT ACT TCC CT TGT	Enterococcus gilvus ATCC BAA-350	144	61°C	40
Clostridium leptum	GCA CAA GCA GTG GAG T CTT CCT CCG TTT TGT CAA	Clostridium leptum ATCC 29065	239	60°C	41
Bacteroides	ATA GCC TTT CGA AAG RAA GAT CCA GTA TCA ACT GCA ATT TTA	Bacteroides vulgatus ATCC 8482	495	60°C	42
Prevotella	CAC RGT AAA CGA TGG ATG CC GGT CGG GTT GCA GAC C	Prevotella nigrescens ATCC 33563	513	55°C	42
Bifidobacterium	CGC GTC YGG TGT GAA AG CCC CAC ATC CAG CAT CCA	Bifidobacterium longum subsp. Infantis ATCC 15697	244	60°C	39

AT, annealing temperature; bp, base pairs.