Table 4.

Quantification of gut microbiota phyla, genera and species in different experimental groups

	Wistar Rat		SD Rat		Two-way ANOVA P values
	St	HF	St	HF	Diet	Strain	Interaction
Firmicutes	6.71 ± 0.06	6.28 ± 0.06 *	6.65 ± 0.03	6.55 ± 0.09 †	0.001	0.118	0.017
Bacteroidetes	6.16 ± 0.11	5.40 ± 0.05	5.55 ± 0.18	5.21 ± 0.12	<0.001	0.004	0.114
Firmicutes/Bacteroidetes	1.09 ± 0.01	1.16 ± 0.01	1.20 ± 0.04	1.26 ± 0.03	0.026	0.001	0.825
Lactobacillus	4.13 ± 0.31	3.56 ± 0.19	5.87 ± 0.12 ‡	2.84 ± 0.29 *	<0.001	0.047	<0.001
Enterococcus	2.65 ± 0.11	2.89 ± 0.08	2.89 ± 0.13	2.87 ± 0.17	0.403	0.410	0.314
Clostridium leptum	5.53 ± 0.07	5.09 ± 0.12 *	5.40 ± 0.06	5.52 ± 0.12 †	0.111	0.126	0.010
Bacteroides	4.07 ± 0.36	4.32 ± 0.12	3.21 ± 0.13	4.05 ± 0.17	0.020	0.017	0.192
Prevotella	4.09 ± 0.32	2.31 ± 0.14 *	2.80 ± 0.25 ‡	2.40 ± 0.14	<0.001	0.015	0.006
Bifidobacterium	2.13 ± 0.11	2.60 ± 0.27	4.33 ± 0.60	3.82 ± 0.37	0.949	<0.001	0.219

Values are presented as mean ± SEM and expressed as log₁₀ 16S rRNA gene copies/20ng of DNA (n = 6 rats per group).

^*P < 0.05 vs respective St diet group,

^‡P < 0.05 between St diet groups and

^†P < 0.05 between HF diet groups.

HF, high-fat diet group; SD, Sprague-Dawley; St, standard diet group.