Table 1.
Comparison of amino acid, acylcarnitine, and succinylacetone quantitation in quality control dried blood spots prepared from pooled human blood with low analyte enrichments (NSQAP specimen 1432).
| NSQAP's in-house Methoda |
Method including C26:0-LPCb |
||||
|---|---|---|---|---|---|
| Analyte | Enrichment, μMc | Mean, μM | 95% C.I., μM | Mean, μM | 95% C.I., μM |
| Phe | 100 | 139.2 | 116.1-162.2 | 146.5 | 124.0-169.0 |
| Leu | 100 | 198.8 | 170.6-227.1 | 195.6 | 140.7-250.4 |
| Met | 50 | 53.1 | 41.4-64.9 | 61.5 | 51.0-72.0 |
| Tyr | 200 | 182.7 | 143.8-221.7 | 203.4 | 165.6-241.1 |
| Val | 200 | 283.2 | 232.8-333.7 | 291.7 | 241.2-342.1 |
| Cit | 25 | 32.3 | 25.5-39.1 | 35.3 | 29.6-41.1 |
| Arg | 100 | 107.2 | 73.8-140.6 | 110.2 | 90.0-130.4 |
| Ala | 200 | 385.6 | 286.4-484.8 | 430.9 | 325.7-536.2 |
| SUAC | 2.5 | 1.4 | 1.2-1.7 | 1.1 | 0.8-1.4 |
| C0 | 10 | 20.1 | 17.0-23.2 | 17.7 | 15.1-20.3 |
| C2 | 10 | 21.1 | 18.0-24.1 | 20.7 | 17.5-23.8 |
| C3 | 3 | 3.7 | 2.6-4.7 | 3.9 | 3.2-4.6 |
| C4 | 1 | 0.9 | 0.7-1.2 | 0.9 | 0.7-1.1 |
| C3DC + C4OH | 1 | 0.3 | 0.2-0.4 | 0.3 | 0.2-0.4 |
| C5 | 0.5 | 0.5 | 0.4-0.7 | 0.5 | 0.4-0.6 |
| C5OH | 0.5 | 1.1 | 0.8-1.4 | 1.3 | 0.9-1.7 |
| C5DC | 0.5 | 0.6 | 0.4-0.9 | 0.6 | 0.4-0.8 |
| C6 | 0.5 | 0.5 | 0.3-0.6 | 0.5 | 0.4-0.6 |
| C8 | 0.5 | 0.5 | 0.4-0.7 | 0.5 | 0.4-0.7 |
| C10 | 0.5 | 0.6 | 0.4-0.8 | 0.6 | 0.4-0.8 |
| C12 | 0.5 | 0.5 | 0.3-0.6 | 0.5 | 0.4-0.6 |
| C14 | 0.5 | 0.5 | 0.4-0.7 | 0.5 | 0.4-0.6 |
| C16 | 3 | 3.1 | 2.3-3.8 | 3.2 | 2.4-3.9 |
| C16OH | 0.1 | 0.1 | 0.0-0.1 | 0.1 | 0.0-0.1 |
| C18 | 1 | 1.5 | 0.9-2.0 | 1.6 | 1.2-2.0 |
| C18OH | 0.5 | 0.2 | 0.1-0.4 | 0.3 | 0.2-0.3 |
| C26:0-LPC | NA | NA | NA | 0.3 | 0.2-0.4 |
a
Results of N = 2 punches on each of 20 days, method of Dhillon, et al.
b
Results of N = 6 punches on each of 7 days, method described in this paper.
c
Enrichment does not include endogenous analytes in the human blood matrix.