Table 2.
Comparison of amino acid, acylcarnitine, and succinylacetone quantitation in quality control dried blood spots prepared from pooled human blood with high analyte enrichments (NSQAP specimen 1434).
| NSQAP's in-house methoda |
Method including C26:0-LPCb |
||||
|---|---|---|---|---|---|
| Analyte | Enrichment, μMc | Mean, μM | 95% C.I., μM | Mean, μM | 95% C.I., μM |
| Phe | 300 | 314.2 | 262.2-366.2 | 343.6 | 301.4-385.8 |
| Leu | 500 | 437.7 | 361.0-514.4 | 447.3 | 347.4-547.2 |
| Met | 250 | 183.9 | 152.7-215.1 | 233.8 | 199.2-268.5 |
| Tyr | 600 | 473.4 | 385.3-561.5 | 548.9 | 466.9-630.9 |
| Val | 500 | 487.0 | 399.5-574.6 | 540.7 | 480.1-601.2 |
| Cit | 250 | 198.4 | 161.7-235.2 | 236.3 | 204.6-268.0 |
| Arg | 300 | 306.9 | 232.5-381.4 | 320.7 | 256.3-385.2 |
| Ala | 600 | 612.4 | 502.6-722.1 | 694.1 | 542.0-846.3 |
| SUAC | 15 | 8.9 | 6.8-11.0 | 6.7 | 5.7-7.7 |
| C0 | 30 | 36.2 | 30.7-41.8 | 35.1 | 31.0-39.1 |
| C2 | 30 | 40.3 | 34.1-46.5 | 42.7 | 37.9-47.5 |
| C3 | 12 | 11.3 | 8.7-13.9 | 12.7 | 10.5-14.9 |
| C4 | 5 | 4.2 | 3.2-5.3 | 4.5 | 3.7-5.4 |
| C3DC + C4OH | 5.5 | 1.1 | 0.8-1.3 | 1.3 | 1.0-1.5 |
| C5 | 3 | 2.7 | 2.0-3.4 | 2.9 | 2.3-3.4 |
| C5OH | 2.5 | 2.9 | 2.1-3.6 | 3.6 | 2.8-4.4 |
| C5DC | 2.5 | 2.6 | 1.7-3.4 | 2.5 | 1.9-3.0 |
| C6 | 2.5 | 2.1 | 1.6-2.6 | 2.5 | 2.0-2.9 |
| C8 | 2.5 | 2.4 | 1.8-2.9 | 2.6 | 2.1-3.2 |
| C10 | 2.5 | 2.5 | 1.9-3.1 | 2.6 | 2.0-3.1 |
| C12 | 2.5 | 2.0 | 1.5-2.6 | 2.5 | 2.0-2.9 |
| C14 | 3 | 2.7 | 2.0-3.4 | 2.7 | 2.2-3.3 |
| C16 | 12 | 9.5 | 7.7-11.3 | 10.5 | 8.8-12.1 |
| C16OH | 1 | 0.5 | 0.3-0.7 | 0.5 | 0.4-0.7 |
| C18 | 5 | 4.6 | 3.6-5.6 | 5.3 | 4.4-6.3 |
| C18OH | 1.5 | 0.7 | 0.3-1.1 | 0.8 | 0.6-1.0 |
| C26:0-LPC | NA | NA | NA | 0.3 | 0.2-0.4 |
a
Results of N = 2 punches on each of 20 different days, method of Dhillon, et al.
b
Results of N = 6 punches on each of 7 different days, method described in this paper.
c
Enrichment does not include endogenous analytes in the human blood matrix.