Fig. 2.

Nestin IR of blood vessels was performed in STC of control 2.1 years. (a, b, c) and ASD donor, age 2.8 years. (d, e). a A low power view showed the nestin-positive arterioles and capillaries in Layer I–VI of the STC in young control. The nestin-positive blood vessels were distributed over all cortical layers. b A view of layer III–V of the STC showed the vessels labeled by the nestin antibody in control (b) and ASD donors (d). Both precapillary arterioles and capillary vessels were labeled. At a higher magnification, the uneven cellular labeling pattern of nestin was apparent in both control (c) and ASD (e) nestin-positive vessels with heavier staining at the junction of the vessels. Nestin-positive glial cells were frequently seen in layer V adjacent to labeled blood vessels in ASD (e) but not NDI controls. Scale bar for a and d = 300 µm and scale bar for c and e is 50 µm