Fig. 3.

Age comparison of nestin IR was made between control and ASD donors. Nestin-positive blood vessels were shown in STC from ASD donors ages 2.8 year (B-6399), 8.5 year (HSB-4640), 14.4 year (UMB-4899) and 20.8 year (UMB-4999). In the NKD control donors, nestin-positive blood vessels were seen at age 2.1 year, (BTB-4235), but not at 8.6 year (UMB-1706), 13.7 year, (UMB-1790) or 20.5 year (UMB-4590). All donors were male except for the youngest control brain, which was a female. In the youngest ASD case (2.8 years), nestin-positive labeling was seen in cells in STC layer V. Scale bar 50 µm