Fig. 5.

Endothelial cell labeling was shown in cerebral blood vessels in an 8.5 year old ASD donor (USB-4640). All antibodies were mouse monoclonal and were used at 1:1000 dilutions (a, b). The label with UEA1 showed endothelial cell bodies on the luminal surface of all blood vessels. UEA1, a lectin, is a specific marker of endothelial cells. There was no cross reactivity to pericytes or glial cells (c, d). The label with CD34 showed endothelial cell bodies and processes on the luminal surface of blood vessels. CD34 is a transmembrane glycoprotein involved in cell–cell adhesion and expressed in hematopoietic progenitor cell antigen found in bone marrow and in developing blood vessels in a variety of organs. Scale bar was 50 µm for a, c and 30 µm for b, d