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. 2016 Mar 7;110(2):238–248. doi: 10.1093/cvr/cvw050

Figure 5.

Figure 5

Adenoviral-mediated overexpression of profilin-1 induces a hypertrophic response in NRVMs. (A) Transcript levels of Pfn1 in NRVMs were significantly higher than control following adenoviral-mediated transfection (n = 12, ***P < 0.001; Student's t-test). (B) Pfn1 overexpression resulted in significantly increased levels of profilin-1 (n = 6, ***P < 0.001; Student's t-test). (C) Elevated Pfn1 expression resulted in increased transcript levels of the hypertrophic markers ANP and BNP (n = 12, *P < 0.05; Student's t-test). (D) NRVMs exhibited significantly larger cellular areas in response to Pfn1 overexpression (n = 33, **P < 0.01; Student's t-test). (E) Representative profilin-1 and α-actinin antibody-stained confocal images of Adv-Control- and Adv-Profilin-1-transfected NRVMs. Scale bar, 15 µm. (F) A ×3.1 zoom of confocal images of Adv-Profilin-1-transfected cells (white box in the merged image). Scale bar, 5 µm.