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. 2016 Mar 7;110(2):238–248. doi: 10.1093/cvr/cvw050

Figure 7.

Figure 7

Profilin-1 is involved in the ERK1/2 signalling pathway. (A) The transcriptional activity of RCAN and MEF2 significantly increased when cells were stimulated with PE. Activity did not decrease upon silencing of profilin-1 (n = 5, **P < 0.01 and ***P < 0.001; two-way ANOVA with the Bonferroni post hoc test). (B) Phosphorylation levels of JNK (corrected for total JNK) and p38 (corrected for total p38) were unaltered by Pfn1 silencing and PE treatment (n = 3, two-way ANOVA with the Bonferroni post hoc test). (C) Phosphorylation of ERK1/2 (corrected for total ERK1/2) and Raf (corrected for GAPDH) was significantly increased in hypertrophic NRVMs and reduced upon diminished profilin-1 expression (n = 3, *P < 0.05 and **P < 0.01; two-way ANOVA with the Bonferroni post hoc test). (D) PE-increased mRNA levels of IL-6 and CTGF, effector genes of the ERK1/2 signalling cascade, were significantly reduced upon silencing of Pfn1 (n = 3, *P < 0.05, **P < 0.01, and ***P < 0.001; two-way ANOVA with the Bonferroni post hoc test).