Figure 1.

Preexposure paradigm. (A) Preexposure paradigm for IOC + Bottle CS delivery experiments. Five-day experimental paradigm where animals receive preexposure to salty and sour tastes or water alone over 2–3 d (dark gray circles days 1–3). Aversions are conditioned on the fourth day across all experiments (white circle day 4). Animals receive bottle and IOC access to sucrose that is immediately paired with LiCl (0.3 M, 0.5% of current body weight) or saline (control). On day 5, aversions are tested by exposure to sucrose via bottle spout for 5 min (light gray circle day 5). Following a 5-min break, animals are then presented with water via lick spout for 5 min. (B) During each preexposure session animals are first presented with 5 min of bottle access to a control substance (left). Following a 5-min intermission, preexposure session animals are presented with salty (N) and sour (C) tastes in addition to water (W) via IOC (right).